Emory University
Contact Info:
Aaron Rae
1760 Haygood Drive
Atlanta, GA 30322
https://www.pedsresearch.org/research/cores/flow-cytometry-core/overview/
Grants and Identifiers:
RRID: RRID:SCR_022324
Instrumentation:
(Equipment No Longer Active At this Facility)
BD Biosciences FACSAria IIu High Speed Cell Sorter
The High Speed Cell Sorter's primary function is to examine complex populations of cells and yield pure populations of cells. [Product Link]
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BD FACSDiscover S8
Cell Sorter, equipped with 5 lasers (355, 405, 488, 561 and 640 nm excitation wavelengths) and 78 channels which can measure the entire emission spectra of the fluorescent dyes, is the first spectral flow cytometer sorter with sort-capable image analysis. [Product Link]
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BD FACSymphony A1 Cell Analyzer
Analyzer applies BD FACSymphony instrument technology to reduce background noise and increase detection sensitivity. Features low-noise electronics, high-powered lasers and tight beam spot to support immunophenotyping up to 16 colors. Enables independent analysis of large and small particles on single instrument. Optional small particle detector enables detection of small particles such as extracellular vesicles, viral particle and more. Compatible with broad portfolio of catalog and custom BD Horizon Brilliant Reagents and BD FACSDiva Software. Permits easy placement on standard lab bench with its compact format. [Product Link]
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BD FACSymphony A3 Cell Analyzer
Fluorescence activated cell analyzing instrument by BD Biosciences. Allows for the simultaneous detection of up to 30 parameters and 28 colors. [Product Link]
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BD FACSymphony A5 Cell Analyzer
Flow cytometer as novel cell analyzer that leverages inherent benefits of flow cytometry and enables simultaneous measurement of up to 50 different characteristics of single cell. [Product Link]
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BD Rhapsody Single-Cell Analysis System
System allows high-throughput capture of multiomic information from single cells using cartridge workflow and multitier barcoding system. Used for high-throughput, multiomic profiling of single cells. Allows to analyze gene expression at both mRNA and protein levels, as well as other cellular characteristics, using microwell-based system with multitiered barcoding approach.This enables the generation of various next-generation sequencing (NGS) libraries for deeper analysis. [Product Link]
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(Equipment No Longer Active At this Facility)
Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
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Cytek Aurora CS system
Advanced and adaptable cell sorting system employs Full Spectrum Profiling technology to produce high-quality flow cytometric data along with Cytek portfolio of full spectrum instruments. Aurora CS system is the only commercially available spectral cell sorter that is paired with companion analyzer. [Product Link]
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
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Luminex Amnis Image Stream Flow Cytometer
ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]
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Other Equipment
BD Biosciences FACSymphony A5 BD Biosciences FACSymphony A3 BD Biosciences FACSymphony A1
Services Provided:
High Dimensional Flow Cytometry Analysis
Relevant Publications:
Description:
The Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several analytical flow cytometers as well as high-speed cell sorting. Training and technical expertise is available to enable our users to improve the quality and scope of their research.
