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Emory + Pediatric/Winship Flow Cytometry Core

Emory University

Contact Info:

Aaron Rae

1760 Haygood Drive

Atlanta, GA 30322

https://www.pedsresearch.org/research/cores/flow-cytometry-core/overview/

Grants and Identifiers:

RRID: RRID:SCR_022324

Instrumentation:

BD Biosciences FACSAria IIu High Speed Cell Sorter

The High Speed Cell Sorter's primary function is to examine complex populations of cells and yield pure populations of cells. [Product Link]

RRID:SCR_019592

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Beckman Coulter CytoFLEX Flow Cytometer

Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]

RRID:SCR_019627

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Cytek Aurora Spectral Analyzer

Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]

RRID:SCR_019826

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Luminex Amnis Image Stream Flow Cytometer

ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]

RRID:SCR_020244

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Other Equipment

BD Biosciences FACSymphony A5 BD Biosciences FACSymphony A3 BD Biosciences FACSymphony A1

Services Provided:

Cell Sorting

FACS Cell Sorting

Flow Cytometric Analysis

High Dimensional Flow Cytometry Analysis

Imaging Flow Cytometry

Spectral Flow Cytometry

Relevant Publications:

1.) Knippler CM, Arnst JL, Robinson IE, Matsuk V, Khatib TO, Harvey RD, Shanmugam M, Mouw JK, Fu H, Ganesh T, Marcus AI (2024 Mar 27). Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion iScience, 27(4), 109591. . PMCID: 11022046.


Description:

The Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several analytical flow cytometers as well as high-speed cell sorting. Training and technical expertise is available to enable our users to improve the quality and scope of their research.