Aarhus University
Charlotte Christie Petersen
Hoegh-Guldbergsgade 10, build. 1116
Aarhus University
Aarhus 8000
https://www.facs.au.dk
RRID: RRID:SCR_022323
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
RRID:SCR_019601
ImageStream-X, Image Analyzer (4 lasers/10 colors) Multiple
ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]
RRID:SCR_020142
BD FACSAria III cell sorter
Agilent NovoCyte 3000
Agilent NovoCyte 2100 YB
Agilent NovoCyte Quanteon 4025
SONY ID7000 full spectrum flow cytometer
Data Analysis
Data Analysis And Interpretation
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
Imaging Flow Cytometry
Multi-color Flow Cytometry
Single-Cell Analysis
Training
1.) van der Sluis RM, Cham LB, Gris-Oliver A, Gammelgaard KR, Pedersen JG, Idorn M, Ahmadov U, Hernandez SS, Cémalovic E, Godsk SH, Thyrsted J, Gunst JD, Nielsen SD, Jørgensen JJ, Bjerg TW, Laustsen A, Reinert LS, Olagnier D, Bak RO, Kjolby M, Holm CK, Tolstrup M, Paludan SR, Kristensen LS, Søgaard OS, Jakobsen MR (2022 May 16). TLR2 and TLR7 mediate distinct immunopathological and antiviral plasmacytoid dendritic cell responses to SARS-CoV-2 infection. The EMBO journal, 41(10), e109622. doi: 10.15252/embj.2021109622. ID: 35178710.
2.) Petersen MA, Rosenberg CA, Brøndum RF, Aggerholm A, Kjeldsen E, Rahbek O, Ludvigsen M, Hasle H, Roug AS, Bill M (2022 May). Immunophenotypically defined stem cell subsets in paediatric AML are highly heterogeneous and demonstrate differences in BCL-2 expression by cytogenetic subgroups. British journal of haematology, 197(4), 452-466. doi: 10.1111/bjh.18094. ID: 35298835.
3.) Staunstrup NH, Petersen CC, Fuglsang T, Starnawska A, Chernomorchenko A, Qvist P, Schack VR (2022 Apr). Comparison of electrostatic and mechanical cell sorting with limited starting material. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 101(4), 298-310. doi: 10.1002/cyto.a.24523. ID: 34842347.
4.) Rossen LS, Schack VR, Thuesen KKH, Bundgaard B, Höllsberg P (2022 Mar 9). CD46 Isoforms Influence the Mode of Entry by Human Herpesvirus 6A/B in T Cells. Journal of virology, 96(5), e0155721. doi: 10.1128/JVI.01557-21. ID: 35019717.
5.) Rosenberg CA, Bill M, Maguire O, Petersen MA, Kjeldsen E, Hokland P, Ludvigsen M (2022 Mar). Imaging flow cytometry reveals a subset of TdT negative T-ALL blasts with very low forward scatter on conventional flow cytometry. Cytometry. Part B, Clinical cytometry, 102(2), 107-114. doi: 10.1002/cyto.b.22035. ID: 34648681.
6.) Konstantin Nissen S, Farmen K, Carstensen M, Schulte C, Goldeck D, Brockmann K, Romero-Ramos M (2022 Mar). Changes in CD163+, CD11b+, and CCR2+ peripheral monocytes relate to Parkinson Brain, behavior, and immunity, 101(), 182-193. . ID: 35026420.
7.) Soerensen JF, Aggerholm A, Rosenberg CA, Bill M, Kerndrup GB, Ebbesen LH, Hansen MH, Roug AS, Ludvigsen M (2022 Mar). Clonal evolution in patients developing therapy-related myeloid neoplasms following autologous stem cell transplantation. Bone marrow transplantation, 57(3), 460-465. doi: 10.1038/s41409-022-01567-z. ID: 35027675.
8.) Simonsen AT, Meggendorfer M, Hansen MH, Nederby L, Koch S, Hansen M, Rosenberg CA, Kern W, Nyvold CG, Aggerholm A, Haferlach T, Ommen HB (2022 Mar). Acute myeloid leukemia exhibiting clonal instability during treatment: Implications for measurable residual disease assessments. Experimental hematology, 107(), 51-59. . ID: 35122908.
9.) Carter-Timofte ME, Arulanandam R, Kurmasheva N, Fu K, Laroche G, Taha Z, van der Horst D, Cassin L, van der Sluis RM, Palermo E, Di Carlo D, Jacobs D, Maznyi G, Azad T, Singaravelu R, Ren F, Hansen AL, Idorn M, Holm CK, Jakobsen MR, van Grevenynghe J, Hiscott J, Paludan SR, Bell JC, Seguin J, Sabourin LA, Côté M, Diallo JS, Alain T, Olagnier D (2021 Nov 12). Antiviral Potential of the Antimicrobial Drug Atovaquone against SARS-CoV-2 and Emerging Variants of Concern. ACS infectious diseases, 7(11), 3034-3051. doi: 10.1021/acsinfecdis.1c00278. ID: 34658235.
10.) Laustsen A, van der Sluis RM, Gris-Oliver A, Hernández SS, Cemalovic E, Tang HQ, Pedersen LH, Uldbjerg N, Jakobsen MR, Bak RO (2021 Sep 2). Ascorbic acid supports ex vivo generation of plasmacytoid dendritic cells from circulating hematopoietic stem cells. eLife, 10(), . pii: e65528. doi: 10.7554/eLife.65528. ID: 34473049.
11.) Rosenberg CA, Bill M, Rodrigues MA, Hauerslev M, Kerndrup GB, Hokland P, Ludvigsen M (2021 Sep). Exploring dyserythropoiesis in patients with myelodysplastic syndrome by imaging flow cytometry and machine-learning assisted morphometrics. Cytometry. Part B, Clinical cytometry, 100(5), 554-567. doi: 10.1002/cyto.b.21975. ID: 33285035.
12.) Petersen MA, Bill M, Rosenberg CA (2021 Apr). OMIP 072: A 15-color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemia. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(4), 382-387. doi: 10.1002/cyto.a.24284. ID: 33369057.
13.) Farmen K, Nissen SK, Stokholm MG, Iranzo A, Østergaard K, Serradell M, Otto M, Svendsen KB, Garrido A, Vilas D, Borghammer P, Santamaria J, Møller A, Gaig C, Brooks DJ, Tolosa E, Pavese N, Romero-Ramos M (2021 Mar 9). Monocyte markers correlate with immune and neuronal brain changes in REM sleep behavior disorder. Proceedings of the National Academy of Sciences of the United States of America, 118(10), . . ID: 33658371.
14.) Schack VR, Rossen LS, Ekebjærg CC, Thuesen KKH, Bundgaard B, Höllsberg P (2021 Jan 27). The tetraspanin protein CD9 modulates infection with human herpesvirus 6A and 6B in a CD46-dependent manner. Journal of virology, (), . . ID: 33504606.
15.) Pedersen JG, Egedal JH, Packard TA, Thavachelvam K, Xie G, van der Sluis RM, Greene WC, Roan NR, Jakobsen MR (2021). Cell-Extrinsic Priming Increases Permissiveness of CD4+ T Cells to Human Immunodeficiency Virus Infection by Increasing C-C Chemokine Receptor Type 5 Co-receptor Expression and Cellular Activation Status. Frontiers in microbiology, 12(), 763030. doi: 10.3389/fmicb.2021.763030. ID: 34899645.
16.) Andersen MN, Skovbo A, Petersen CC, Hokland M (2020 Sep). Application Settings versus Fixed Photomultiplier Tube Voltages for Optimal Cytometer Stability over Time, and Effect on Reusing a Compensation Matrix. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(9), 965-974. doi: 10.1002/cyto.a.24013. ID: 32314518.
17.) Hagert CF, Bohn AB, Wittenborn TR, Degn SE (2020 Aug). Seeing the Confetti Colors in a New Light Utilizing Flow Cytometry and Imaging Flow Cytometry. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(8), 811-823. doi: 10.1002/cyto.a.24032. ID: 32459058.
18.) Simonsen AT, Bill M, Rosenberg CA, Hansen MH, Møller PL, Kjeldsen E, Johansen KD, Ommen HB, Nederby L, Aggerholm A, Hokland P, Ludvigsen M (2020 May). Unraveling clonal heterogeneity at the stem cell level in myelodysplastic syndrome: In pursuit of cell subsets driving disease progression. Leukemia research, 92(), 106350. . ID: 32334198.
19.) Pedersbæk D, Jønsson K, Madsen DV, Weller S, Bohn AB, Andresen TL, Simonsen JB (2020 Jan 22). A quantitative <i>ex vivo</i> study of the interactions between reconstituted high-density lipoproteins and human leukocytes. RSC advances, 10(7), 3884-3894. doi: 10.1039/c9ra08203d. ID: 35492676.
20.) Alstrup T, Eijken M, Bohn AB, Møller B, Damsgaard TE (2019 Feb). Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. Current protocols in stem cell biology, 48(1), e68. doi: 10.1002/cpsc.68. ID: 30365239.
21.) Haslund D, Ryø LB, Seidelin Majidi S, Rose I, Skipper KA, Fryland T, Bohn AB, Koch C, Thomsen MK, Palarasah Y, Corydon TJ, Bygum A, Nejsum LN, Mikkelsen JG (2019 Jan 2). Dominant-negative SERPING1 variants cause intracellular retention of C1 inhibitor in hereditary angioedema. The Journal of clinical investigation, 129(1), 388-405. . ID: 30398465.
22.) Hansen AS, Biltoft M, Bundgaard B, Bohn AB, Møller BK, Höllsberg P (2019 Jan). CD46 activation induces distinct CXCL-10 response in monocytes and monocyte-derived dendritic cells. Cytokine, 113(), 466-469. . ID: 29980471.
23.) Hansen AS, Slater J, Biltoft M, Bundgaard BB, Møller BK, Höllsberg P (2018 Aug). CD46 is a potent co-stimulatory receptor for expansion of human IFN-γ-producing CD8<sup>+</sup> T cells. Immunology letters, 200(), 26-32. . ID: 29902483.
24.) Kristensen AM, Stengaard-Pedersen K, Hetland ML, Hørslev-Petersen K, Junker P, Østergaard M, Höllsberg P, Deleuran B, Hvid M (2017 Aug). Expression of soluble CD83 in plasma from early-stage rheumatoid arthritis patients is not modified by anti-TNF-α therapy. Cytokine, 96(), 1-7. . ID: 28267648.
25.) Hansen AS, Bundgaard BB, Biltoft M, Rossen LS, Höllsberg P (2017 Feb). Divergent tropism of HHV-6A<sub>GS</sub> and HHV-6B<sub>PL1</sub> in T cells expressing different CD46 isoform patterns. Virology, 502(), 160-170. . ID: 28056415.
The FACS Core Facility at Aarhus University is an open fee-for-service SRL. The facility has 3 highly skilled staff, taking care of training and maintenance. The facility is equipped with 2 cell sorters, 4 analyzers, 1 ImageStream and 1 full spectrum flow cytometer.
