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Center for Host-Microbial Interactions

University of Pennsylvania

Contact Info:

Daniel Beiting

380 S. University Avenue

Hill Pavilion

Philadelphia, PA 19104

https://hostmicrobe.org/

Grants and Identifiers:

RRID: RRID:SCR_022310

Instrumentation:

Agilent 4200 TapeStation System

TapeStation System is used to analyze DNA and RNA samples for quality control. [Product Link]

RRID:SCR_019398

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Illumina MiniSeq Next Generation Sequencer

Illumina NGS technology performs broad range of targeted DNA and RNA applications. [Product Link]

RRID:SCR_020136

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Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems

Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]

RRID:SCR_020140

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(Equipment No Longer Active At this Facility)

Nanodrop Qubit 3 Fluorometer

Benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit quantitation assays. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3.0 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer. You can also use the Qubit 3.0 Fluorometer to directly measure the fluorescence of samples or to create new assay(s) using the MyQubit software pre-programmed into the instrument. [Product Link]

RRID:SCR_020311

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Services Provided:

10x Genomics

ATAC-Seq

ChIP-Seq

CITE-seq

Consultations

Data Processing

DNA Analysis

DNA Isolation

Genomics

Long Read Sequencing

Metagenomics Analysis

Microbiome

Nucleic Acid Extraction

Pyrosequencing

Real-time qPCR

RNA Integrity

RNA Isolation

RNAseq

Sequencing - DNA Sequencing

Sequencing - Next Generation Sequencing (NGS)

Sequencing - Pyrosequencing

Single-Cell Analysis

Single-cell Sequencing

Training

Whole Genome Sequencing

Relevant Publications:

1.) Pardy RD, Walzer KA, Wallbank BA, Byerly JH, O'Dea KM, Cohn IS, Haskins BE, Roncaioli JL, Smith EJ, Buenconsejo GY, Striepen B, Hunter CA (2024 May). Analysis of intestinal epithelial cell responses to Cryptosporidium highlights the temporal effects of IFN-γ on parasite restriction. PLoS pathogens, 20(5), e1011820. . PMID: 38718306.


Description:

We study the biological basis of diseases caused by microbes – whether it be a parasitic worm, a pathogenic bacterium, or a complex microbial community in the gut. Our group makes up the Center for Host-Microbial Interactions, at PennVet, and our research leverages a diverse skill set that cuts across the disciplines of genomics, microbiology and immunology. We engage in collaborative projects that benefit from close interactions with veterinarians and human clinicians alike. Our research embodies the idea of One-Health - that humans, animals and the environment are interconnected, and that we all live in a microbial world. We are located at the University of Pennsylvania School of Veterinary Medicine, in the Department of Pathobiology.