University of Ottawa
Vera Tang
451 Smyth Rd.
Ottawa, ON K1H5M8
https://www.uottawa.ca/research-innovation/flow-cytometry-virometry
RRID: RRID:SCR_023306
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
RRID:SCR_019601
Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
RRID:SCR_019627
Sony Biotechnology SH800S Cell Sorter
The benchtop SH800S cell sorter is a cell sorter that sorts a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective and easier to perform. System software supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product. The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. [Product Link]
RRID:SCR_018066
Cytek Aurora Spectral Analyzer ESP
5 laser configuration
Cytek Northern Lights
Spectral Analyzer
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
Single Particle Extracellular Vesicle Flow Cytometry
Single Particle Flow Virometry
1.) Akuma M, Kim M, Zhu C, Wiljer E, Gaudreau-Lapierre A, Patterson LD, Egevad L, Tanguay S, Trinkle-Mulcahy L, Stanford WL, Riazalhosseini Y, Russell RC (2025 Apr 16). Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma Cell Death & Disease, 16(1), 307. . PMCID: 12003641.
2.) Fernandes C, Persaud AT, Chaphekhar D, Burnie J, Belanger C, Tang VA, Guzzo C (2025 Feb 25). Flow virometry: recent advancements, best practices, and future frontiers. Journal of virology, 99(2), e0171724. . ID: 39868829.
3.) Minarrieta L, Annis MG, Audet-Delage Y, Kuasne H, Pacis A, St-Louis C, Nowakowski A, Biondini M, Khacho M, Park M, Siegel PM, St-Pierre J (2024 Nov 6). Mitochondrial elongation impairs breast cancer metastasis Science Advances, 10(45), eadm8212. . PMCID: 11540020.
4.) Smith TKT, Ghorbani P, LeBlond ND, Nunes JRC, O'Dwyer C, Ambursley N, Fong-McMaster C, Minarrieta L, Burkovsky LA, El-Hakim R, Trzaskalski NA, Locatelli CAA, Stotts C, Pember C, Rayner KJ, Kemp BE, Loh K, Harper ME, Mulvihill EE, St-Pierre J, Fullerton MD (2024 Oct). AMPK-mediated regulation of endogenous cholesterol synthesis does not affect atherosclerosis in a murine Pcsk9-AAV model. Atherosclerosis, 397(), 117608. doi: 10.1016/j.atherosclerosis.2024.117608. PMID: 38880706.
5.) Burnie J, Fernandes C, Patel A, Persaud AT, Chaphekar D, Wei D, Lee TKH, Tang VA, Cicala C, Arthos J, Guzzo C (2024 Jun 9). Applying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences Across HIV Model Systems. Viruses, 16(6), . . ID: 38932227.
6.) Nunes JRC, O'Dwyer C, Ghorbani P, Smith TKT, Chauhan S, Robert-Gostlin V, Girouard MD, Viollet B, Foretz M, Fullerton MD (2024 Jun). Myeloid AMPK signaling restricts fibrosis but is not required for metformin improvements during CDAHFD-induced NASH in mice. Journal of lipid research, 65(6), 100564. . PMID: 38762124.
7.) Persaud AT, Khela J, Fernandes C, Chaphekar D, Burnie J, Tang VA, Colpitts CC, Guzzo C (2024 May 14). Virion-incorporated CD14 enables HIV-1 to bind LPS and initiate TLR4 signaling in immune cells. Journal of virology, 98(5), e0036324. . ID: 38661384.
8.) Belkina AC, Roe CE, Tang VA, Back JB, Bispo C, Conway A, Chakraborty U, Daniels KT, de la Cruz G, Ferrer-Font L, Filby A, Gravano DM, Gregory MD, Hall C, Kukat C, Mozes A, Ordoñez-Rueda D, Orlowski-Oliver E, Pesce I, Porat Z, Poulton NJ, Reifel KM, Rieger AM, Sheridan RTC, Van Isterdael G, Walker RV (2024 Feb). Guidelines for establishing a cytometry laboratory. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 105(2), 88-111. doi: 10.1002/cyto.a.24807. ID: 37941128.
9.) Welsh JA, Goberdhan DCI, O (2024 Feb). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of extracellular vesicles, 13(2), e12404. . ID: 38326288.
10.) Galpin KJ, Rodriguez GM, Maranda V, Cook DP, Macdonald E, Murshed H, Zhao S, McCloskey CW, Chruscinski A, Levy GA, Ardolino M, Vanderhyden BC (2024 Jan 8). FGL2 promotes tumour growth and attenuates infiltration of activated immune cells in melanoma and ovarian cancer models Scientific Reports, 14(), 787. . PMCID: 10774293.
11.) Li J, Vranjkovic A, Read D, Delaney SP, Stanford WL, Cooper CL, Crawley AM (2024). Lasting differential gene expression of circulating CD8 T cells in chronic HCV infection with cirrhosis identifies a role for Hedgehog signaling in cellular hyperfunction. Frontiers in immunology, 15(), 1375485. . PMID: 38887299.
12.) Burnie J, Fernandes C, Chaphekar D, Wei D, Ahmed S, Persaud AT, Khader N, Cicala C, Arthos J, Tang VA, Guzzo C (2023 Dec 27). Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay. Scientific reports, 13(1), 23025. . ID: 38155248.
13.) Cook S, Tang VA, Lannigan J, Jones JC, Welsh JA (2023 Dec 18). Quantitative flow cytometry enables end-to-end optimization of cross-platform extracellular vesicle studies. Cell reports methods, 3(12), 100664. . ID: 38113854.
14.) Farokhi Boroujeni S, Rodriguez G, Galpin K, Yakubovich E, Murshed H, Ibrahim D, Asif S, Vanderhyden BC (2023 Nov 28). BRCA1 and BRCA2 deficient tumour models generate distinct ovarian tumour microenvironments and differential responses to therapy Journal of Ovarian Research, 16(), 231. . PMCID: 10683289.
15.) Pleet ML, Cook S, Tang VA, Stack E, Ford VJ, Lannigan J, Do N, Wenger E, Fraikin JL, Jacobson S, Jones JC, Welsh JA (2023 Oct 25). Extracellular Vesicle Refractive Index Derivation Utilizing Orthogonal Characterization. Nano letters, 23(20), 9195-9202. . ID: 37788377.
16.) Ghorbani P, Kim SY, Smith TK, Minarrieta L, Robert-Gostlin V, Kilgour MK, Ilijevska M, Alecu I, Snider SA, Margison KD, Nunes JR, Woo D, Pember C, O’Dwyer C, Ouellette J, Kotchetkov P, St-Pierre J, Bennett SA, Lacoste B, Blais A, Nair MG, Fullerton MD (2023 Sep 25). Choline metabolism underpins macrophage IL-4 polarization and RELMα up-regulation in helminth infection PLOS Pathogens, 19(9), e1011658. . PMCID: 10553840.
17.) Welsh JA, Arkesteijn GJA, Bremer M, Cimorelli M, Dignat-George F, Giebel B, Görgens A, Hendrix A, Kuiper M, Lacroix R, Lannigan J, van Leeuwen TG, Lozano-Andrés E, Rao S, Robert S, de Rond L, Tang VA, Tertel T, Yan X, Wauben MHM, Nolan JP, Jones JC, Nieuwland R, van der Pol E (2023 Feb). A compendium of single extracellular vesicle flow cytometry. Journal of extracellular vesicles, 12(2), e12299. . ID: 36759917.
18.) Rodriguez GM, Yakubovich E, Murshed H, Maranda V, Galpin KJC, Cudmore A, Hanna AMR, Macdonald E, Ramesh S, Garson K, Vanderhyden BC (2023). NLRC5 overexpression in ovarian tumors remodels the tumor microenvironment and increases T-cell reactivity toward autologous tumor-associated antigens. Frontiers in immunology, 14(), 1295208. . PMID: 38235131.
19.) Burnie J, Persaud AT, Thaya L, Liu Q, Miao H, Grabinsky S, Norouzi V, Lusso P, Tang VA, Guzzo C (2022 May 21). P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is incorporated into clinical HIV-1 isolates and can mediate virus capture and subsequent transfer to permissive cells. Retrovirology, 19(1), 9. . ID: 35597982.
20.) Welsh JA, Tang VA, van der Pol E, Görgens A (2021 Apr). MIFlowCyt-EV: The Next Chapter in the Reporting and Reliability of Single Extracellular Vesicle Flow Cytometry Experiments. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(4), 365-368. doi: 10.1002/cyto.a.24268. ID: 33200505.
21.) Aspland AM, Douagi I, Filby A, Jellison ER, Martinez L, Shinko D, Smith AL, Tang VA, Thornton S (2021 Jan). Biosafety during a pandemic: shared resource laboratories rise to the challenge. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(1), 68-80. . ID: 33289290.
22.) Burnie J, Tang VA, Welsh JA, Persaud AT, Thaya L, Jones JC, Guzzo C (2020 Nov 12). Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles. Viruses, 12(11), . . ID: 33198254.
23.) Welsh JA, Jones JC, Tang VA (2020 Jun). Fluorescence and Light Scatter Calibration Allow Comparisons of Small Particle Data in Standard Units across Different Flow Cytometry Platforms and Detector Settings. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(6), 592-601. . ID: 32476280.
24.) Renner TM, Tang VA, Burger D, Langlois MA (2020 Jan 6). Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus. Journal of virology, 94(2), . . ID: 31694951.
25.) Czechowska K, Lannigan J, Aghaeepour N, Back JB, Begum J, Behbehani G, Bispo C, Bitoun D, Fernández AB, Boova ST, Brinkman RR, Ciccolella CO, Cotleur B, Davies D, Dela Cruz GV, Del Rio-Guerra R, Des Lauriers-Cox AM, Douagi I, Dumrese C, Bonilla Escobar DL, Estevam J, Ewald C, Fossum A, Gaudillière B, Green C, Groves C, Hall C, Haque Y, Hedrick MN, Hogg K, Hsieh EWY, Irish J, Lederer J, Leipold M, Lewis-Tuffin LJ, Litwin V, Lopez P, Nasdala I, Nedbal J, Ohlsson-Wilhelm BM, Price KM, Rahman AH, Ra (2019 Dec). Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2019 Conference Workshops. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(12), 1236-1274. doi: 10.1002/cyto.a.23941. ID: 31833655.
26.) Brittain GC 4th, Chen YQ, Martinez E, Tang VA, Renner TM, Langlois MA, Gulnik S (2019 Nov 5). A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles. Scientific reports, 9(1), 16039. . ID: 31690751.
27.) Ruzicka M, Xiao F, Abujrad H, Al-Rewashdy Y, Tang VA, Langlois MA, Sorisky A, Ooi TC, Burger D (2019 Aug 2). Effect of hemodialysis on extracellular vesicles and circulating submicron particles. BMC nephrology, 20(1), 294. . ID: 31375072.
28.) Tang VA, Renner TM, Fritzsche AK, Burger D, Langlois MA (2017 Dec 19). Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry. Scientific reports, 7(1), 17769. . ID: 29259315.
29.) Tang VA, Renner TM, Varette O, Le Boeuf F, Wang J, Diallo JS, Bell JC, Langlois MA (2016 Sep 30). Single-particle characterization of oncolytic vaccinia virus by flow virometry. Vaccine, 34(42), 5082-5089. doi: 10.1016/j.vaccine.2016.08.074. ID: 27614781.
We are a flow cytometry core facility located at the Faculty of Medicine providing cutting edge instrumentation and expertise to researchers in Ottawa and around the world. We specialize in single-particle analysis of viruses and extracellular vesicles.
