University of California, Santa Cruz
Bari Holm Nazario
1156 High Street
Biomed 450
Santa Cruz, CA 95064
https://ibsc.ucsc.edu/facilities/cytometry
RRID: RRID:SCR_021149
BD FACSAria Aria I Flow Cytometer
BD FACSAria cell sorter incorporates a fixed-alignment cuvette flow cell that can detect up to 13 colors, for a total of 15parameters. [Product Link]
RRID:SCR_019595
BD FACS LSRII SORP
FACS Cell Sorting
Multi-color Flow Cytometry
1.) Yang C, Rodriguez Y Baena A, Manso BA, Hu S, Lopez-Magaña R, Ohanyan M, Ottemann KM (2025 Jan 7). Helicobacter pylori luxS mutants cause hyperinflammatory responses during chronic infection. Microbiology spectrum, 13(1), e0107324. . PMID: 39641542.
2.) Manso BA, Medina P, Smith-Berdan S, Baena ARY, Bachinsky E, Mok L, Deguzman A, Avila SB, Chattopadhyaya S, Rommel MGE, Jönsson VD, Forsberg EC (2024 Nov 5). A rare HSC-derived megakaryocyte progenitor accumulates via enhanced survival and contributes to exacerbated thrombopoiesis upon aging. bioRxiv : the preprint server for biology, (), . . PMID: 39574585.
3.) Poscablo DM, Worthington AK, Smith-Berdan S, Rommel MGE, Manso BA, Adili R, Mok L, Reggiardo RE, Cool T, Mogharrab R, Myers J, Dahmen S, Medina P, Beaudin AE, Boyer SW, Holinstat M, Jonsson VD, Forsberg EC (2024 Jun 6). An age-progressive platelet differentiation path from hematopoietic stem cells causes exacerbated thrombosis. Cell, 187(12), 3090-3107.e21. doi: 10.1016/j.cell.2024.04.018. PMID: 38749423.
4.) Barrett AK, Shingare MR, Rechtsteiner A, Rodriguez KM, Le QN, Wijeratne TU, Mitchell CE, Membreno MW, Rubin SM, Müller GA (2024 May 24). HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexes. Nature communications, 15(1), 4450. . PMID: 38789411.
5.) Martin EW, Rodriguez Y Baena A, Reggiardo RE, Worthington AK, Mattingly CS, Poscablo DM, Krietsch J, McManus MT, Carpenter S, Kim DH, Forsberg EC (2023 May 15). Dynamics of Chromatin Accessibility During Hematopoietic Stem Cell Differentiation Into Progressively Lineage-Committed Progeny. Stem cells (Dayton, Ohio), 41(5), 520-539. . PMID: 36945732.
6.) Johnson KS, Yang C, Carter JE, Worthington AK, Robinson EK, Lopez-Magaña R, Salgado F, Arnold I, Ottemann KM (2023 Jan 24). Helicobacter pylori Chronic-Stage Inflammation Undergoes Fluctuations That Are Altered in tlpA Mutants. Infection and immunity, 91(1), e0032222. . PMID: 36533917.
7.) Worthington AK, Cool T, Poscablo DM, Hussaini A, Beaudin AE, Forsberg EC (2022 Apr 15). IL7Rα, but not Flk2, is required for hematopoietic stem cell reconstitution of tissue-resident lymphoid cells. Development (Cambridge, England), 149(8), . . PMID: 35072209.
8.) Robinson EK, Worthington A, Poscablo D, Shapleigh B, Salih MM, Halasz H, Seninge L, Mosqueira B, Smaliy V, Forsberg EC, Carpenter S (2022 Apr 15). lincRNA-Cox2 Functions to Regulate Inflammation in Alveolar Macrophages during Acute Lung Injury. Journal of immunology (Baltimore, Md. : 1950), 208(8), 1886-1900. . PMID: 35365562.
9.) Rodriguez Y Baena A, Rajendiran S, Manso BA, Krietsch J, Boyer SW, Kirschmann J, Forsberg EC (2022 Feb 24). New transgenic mouse models enabling pan-hematopoietic or selective hematopoietic stem cell depletion in vivo. Scientific reports, 12(1), 3156. . PMID: 35210475.
10.) Cazares O, Chatterjee S, Lee P, Strietzel C, Bubolz JW, Harburg G, Howard J, Katzman S, Sanford J, Hinck L (2021 Nov 1). Alveolar progenitor differentiation and lactation depends on paracrine inhibition of notch via ROBO1/CTNNB1/JAG1. Development (Cambridge, England), 148(21), . . PMID: 34758082.
11.) Smith-Berdan S, Bercasio A, Kramer L, Petkus B, Hinck L, Forsberg EC (2021). Acute and endothelial-specific Robo4 deletion affect hematopoietic stem cell trafficking independent of VCAM1. PloS one, 16(8), e0255606. doi: 10.1371/journal.pone.0255606. PMID: 34388149.
The FACS facility provides user training for self-op use of BD FACS LSRII analyzer 3-laser and Self-op and Assisted sorting on BD FACS Aria IIU 5-lasers
