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Multiscale Microscopy Core

Oregon Health & Science University

Contact Info:

Claudia Lopez

2730 S. Moody Ave

Portland, OR 97201

https://www.ohsu.edu/multiscale-microscopy-core

Grants and Identifiers:

RRID: RRID:SCR_022652

Instrumentation:

FEI Glacios Cryo-TEM (Transmission Electron Microscope) for Life Sciences

Microscope that features 200 kV XFEG optics and ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. [Product Link]

RRID:SCR_019883

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FEI Volumescope 2 SEM

Microscope that is used for large volume samples with serial block face imaging and multi energy deconvolution. [Product Link]

RRID:SCR_019913

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Leica EM AFS2 Freeze Substitution Device

Leica EM AFS2 is a freeze substitution platform that performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use. [Product Link]

RRID:SCR_020230

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Other Equipment

- FEI Tecnai™ T12 Cameras: Eagle and AMT NanoSprint12S-B cMOS - Helios G3 FIB-SEM -Helios UC5 FIB-SEM - Leica UC7 Cryo-Ultramicrotome - Leica 3D ARTOS Ultramicrotome - Leica ACE600 Metal/Carbon Coater - Leica ACE600 cryo-Coater with e-Beam deposition - Thermo Scientific Vitrobot Mk IV - Wohlwend High-pressure Freezer -Thermo Scientific CorrSight

Services Provided:

Cryo-Electron Microscopy

Electron Microscopy

Focused Ion Beam (FIB)

Freeze Substitution

High-Pressure Freezing

Immuno Electron Microscopy

Sample Preparation

Scanning Electron Microscopy

Transmission Electron Microscopy

Relevant Publications:

1.) Shi X, Palumbo MC, Benware S, Wiedrick J, Markwardt S, Janowsky A (2025 Jun 20). Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory Study bioRxiv, (), 2025.06.16.659918. . PMCID: 12262688.


2.) Garrood M, Thorn EL, Goldstein A, Sowa A, Janssen W, Wilson A, López CS, Shankar R, Stempinski ES, Farrell K, Crary JF, McKenzie AT (2025 Feb 4). Preservation of cellular structure via immersion fixation in brain banking Free Neuropathology, 6(), 4. . PMCID: 11795511.


Description:

The Multiscale Microscopy Core at OHSU is a state-of-the-art electron microscopy core that provides services and training to academic and corporate users.