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Metabolite Profiling Facility

Purdue University West Lafayette

Contact Info:

Natasha Nikolaidis

1203 West State St.

West Lafayette, IN 47906

https://www.purdue.edu/discoverypark/bioscience/facilities/core/metabolite/index.php

Grants and Identifiers:

RRID: RRID:SCR_022728

Instrumentation:

ABI Voyager DE Pro MALDI-Mass Spectrometer

Benchtop MALDI TOF mass spectrometer that has linear and reflector analyzer. It is used to analyze organic and inorganic compounds, protein identification, synthetic peptide validation, and polymer analysis. [Product Link]

RRID:SCR_018432

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Agilent 6470 Triple Quadrupole Liquid Chromatography Mass Spectrometer System

Triple quadrupole LC/MS system for commercial, regulatory, life sciences, and research applications. It has Agilent Jet Stream ion source and curved geometry collision cell. It detects target compounds at low levels in diverse matrices, while achieving wide linear dynamic range. [Product Link]

RRID:SCR_019421

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Agilent 6545 LC/Q-TOF

LF/Q-TOF is used for untargeted sample analysis and enables broad screening, comprehensive profiling, and identification of unknowns. [Product Link]

RRID:SCR_019429

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Waters Synapt G2 Mass Spectrometer

Synapt G2 Mass Spectrometry (MS) system is a hybrid, quadrapole/orthogonal acceleration, time-of-flight (oa-TOF) mass spectrometer controlled by MassLynx software. The Waters Synapt G2 is compatible with the Acquity UPLC and nanoAcquity UPLC systems. Hamilton STARplus liquid handling robot. [Product Link]

RRID:SCR_020880

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Other Equipment

Agilent 6460 Triple Quadrupole LC/MS/MS Agilent 6410 Triple Quadrupole LC/MS Agilent 6550 LC/Q-TOF Thermo Scientific TSQ 8000 GC/MS/MS Waters Synapt XS Mass Spectrometer

Services Provided:

Amino Acid Analysis

Consultation

Consultations

Data Analysis

Data Analysis And Interpretation

Data Processing

Data Processing And Interpretation

GC

Imaging Mass Spectrometry

Lipidomics

Mass Spectrometry

Mass Spectrometry - Small Molecules

Metabolomics

Training

Relevant Publications:

Description:

The Metabolite Profiling Facility (MPF) provides state-of-the-art technologies that enable both qualitative (defining all components of a metabolome) and quantitative (determining differential concentrations of metabolites) metabolomics in complex biological systems. This facility employs highly sensitive mass spectrometry coupled with liquid chromatography and gas chromatography for precise sample analysis. Our scientists empower researchers with new technologies, methods development, sample analysis, expert training, and consultation.