Core provides specialized expertise, instrumentation, and technical support for assessment of mitochondrial function in cells, tissues, and isolated mitochondria. Provides standardized mitochondrial isolation, experimental design consultation, assay optimization, data analysis, and interpretation. Services include high-resolution respirometry using the Oroboros O2k-FluoRespirometer, simultaneous measurement of oxygen consumption and reactive oxygen species production, mitochondrial respiratory control and coupling analyses, electron transport system complex activity assays, citrate synthase activity, mitochondrial calcium handling, and assessment of mitochondrial content and oxidative stress. Studies can be performed using isolated mitochondria, permeabilized tissue fibers, tissue homogenates, and intact cells, depending on the scientific question and sample availability. Core also assists with protocol development, selection of normalization strategies, troubleshooting, training, and preparation of methods and data for grants, presentations, and publications.

Facility promotes and facilitates faculty and student research in biomedical bench science. Provides supports for clinical research, expertise and resources to clinical and translational scientists and trainees. Offers services of clinical research coordinators and BSL2 facilities for the processing, storage or molecular experimentation of human biological samples. In addition, equipment and technical assistance for performing ELISAs, cell proliferation assays, autoantibody assays, nitric oxide (NO) measurements, reactive oxygen species (ROS) determination, proteomics, mitochondrial function, flow cytometry, Western immunoblotting, and cell cytokine secretion profiling are available.