The CoreMarketplace: CUNY Advanced Science Research Center-Epigenetics Core

ASRC-Live Imaging and Bioenergetics Core (Imaging (Cell, Molecular, PET, Translational)) RRID:SCR_022945	CUNY Advanced Science Research Center New York, NY 10031		Last Updated: 11/01/2022
CUNY Advanced Science Research Center-Epigenetics Core (Epigenomics) RRID:SCR_021914	CUNY Advanced Science Research Center New York, NY 10031	10x Genomics, FACS Cell Sorting, DNA Isolation, Multi-color Flow Cytometry, Single-cell Sequencing, Sequencing - Next Generation Sequencing (NGS), RNAseq, In Situ Hybridization, RNA Isolation, Real-time qPCR, Cell Sorting, Cel	Last Updated: 01/11/2022

CUNY Advanced Science Research Center

85 Saint Nicholas Terrace

New York, NY 10031

United States of America

https://asrc.gc.cuny.edu/neuroscience/facilities/epigenetics-core-facility/

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Quicklinks:

https://coremarketplace.org/asrcepigeneticscore https://coremarketplace.org/RRID:SCR_021914

Primary Contact:

Jia Liu

Last Updated: 01/11/2022

Facility RRID

RRID:SCR_021914

Facility Details

The Epigenetics Core Facility at the Advanced Science Research Center of City University of New York provides a variety of research resources and services that include: nucleic acid extraction and fragmentation, quantitative gene expression analysis, targeted DNA methylation analysis in specific genomic loci of interest, next generation sequencing (RNA-seq and ChIP-seq), and single cell genomics. The facility also houses an advanced cell sorter with biosafety protection for flow cytometry service. In addition, the facility provides services for RNAScope to allow for in situ hybridization detection of transcriptional targets with spatial resolution. The facility is open to both academic and non-academic users.

Facility Policies

Services are offerred outside of CUNY Advanced Science Research Center

Consulting is offerred outside of CUNY Advanced Science Research Center

Services offered:

In Situ Hybridization

Multi-color Flow Cytometry

Real-time qPCR

RNA Isolation

RNAseq

Sequencing - Next Generation Sequencing (NGS)

Single-cell Sequencing

Facility Equipment

Agilent 4200 Tapestation Genetic Analyzer

Benchtop genetic analyzer electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow. [Product Link]

RRID:SCR_018435

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Fluidigm C1 Single-Cell Automated Preparation System

Automated preparation system that isolates and prepares single cell templates for analysis. It can analyze mRNA and DNA sequencing, epigenetics or miRNA expression. It can extract, reverse transcribe, amplify, and analyze gene expression. [Product Link]

RRID:SCR_018578

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Life Technologies QuantStudio 7 Real Time PCR System

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, software, automation capabilities, and touch-screen interface, the QuantStudio 7 Flex system offers reproducibility with minimal well-to-well and instrument-to-instrument variation. [Product Link]

RRID:SCR_020245

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Nanodrop Qubit 3 Fluorometer

Benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit quantitation assays. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3.0 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer. You can also use the Qubit 3.0 Fluorometer to directly measure the fluorescence of samples or to create new assay(s) using the MyQubit software pre-programmed into the instrument. [Product Link]

RRID:SCR_020311

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Pippin Prep

Facilitates library construction system for NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. [Product Link]

RRID:SCR_020403

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10X GenomicsChromium iX Controller
Agena MassArray System
Fluidigm Biomark HD
ACD RNAscope
BD AriaFusion cell sorter

Publications

No publications have been associated with this facility

Associations

Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=1262
https://coremarketplace.org/RRID:SCR_021914

Institution

Institution ROR ID: https://ror.org/01gdjt538

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, epigenetics, nucleic acid extraction and fragmentation, quantitative gene expression analysis, targeted DNA methylation analysis in specific genomic loci of interest, next generation sequencing, RNA-seq, ChIP-seq, single cell genomics

Resource Type:

access service resource, service resource, core facility

Citation:

City University of New York Advanced Science Research Center Epigenetics Core Facility (RRID:SCR_021914)

CUNY Advanced Science Research Center-Epigenetics Core (Epigenomics)

Quicklinks:

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

Contact Jia Liu

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CUNY Advanced Science Research Center-Epigenetics Core

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