RRID:SCR_024711 | Lincoln, NE 68583 | Cell Sorting, Consultations, Multi-color Flow Cytometry, Exosomes Characterisation, Experimental Design, FACS Cell Sorting, Flow Cytometric Analysis, Flow Cytometry, Flow Cytometry Data Analysis, Staining, Single Particle Extracellular Vesicle Flow Cytometry, Sample Preparation | 03/04/2024 |
RRID:SCR_025012 | Lincoln, NE 68583 | Cryo-Electron Microscopy, Cryo-Electron Tomography, Data Analysis, Data Processing, MicroED | 02/13/2024 |
RRID:SCR_025011 | Lincoln, NE 68588 | Computational - Bioinformatics, Computational - Biostatistics, Computational - High Performance Computing, Computational - Interactive Compute/storage, Computational - Application Development, Consultation, Consultations, Data Analysis, Data Analysis And Interpretation, Data Processing, Data Processing And Interpretation, Data Visualization, Genome/Transcriptome Assembly And Annotation, Genomics, High Performance Computing, Metagenomics Analysis, Methylation Analysis, Metatranscriptomics, Microbiome, Pipeline Development, Power Analysis, Proteomics Analysis, Quantitative DNA Sequence Mapping, RNA analysis, Single-Cell Analysis, Spatial / Single Cell Data Analysis, Spatial Transcriptomics | 02/13/2024 |
RRID:SCR_021314 | Lincoln, NE 68588 | Amino Acid Analysis, Assay Development, Customized Protocols, GC, HPLC, HRAM Mass Spectrometry, Mass Spectrometry, Metabolomics, Phosphoproteomics, Protein Extraction/Purification, Proteomics, Sample Preparation, Data Analysis, SDS-PAGE, Untargeted Mass Spectrometry | 06/23/2021 |
RRID:SCR_017798 | Lincoln, NE 68588 | Cell Imaging, Confocal Microscopy, Electron Microscopy, Immunohistochemistry, Laser Capture Microdissection, Microscopy | 07/01/2015 |
University of Nebraska–Lincoln
Beadle E154
1901 Vine St.
Lincoln, NE 68588
United States of Americahttps://biotech.unl.edu/proteomics-and-metabolomics#tab1cite this facility
Sophie Alvarez
Last Updated: 06/23/2021
The Proteomics and Metabolomics Facility (PMF) at the Center for Biotechnology was established in 2015 under the leadership of the Director, Dr. Sophie Alvarez and the Assistant Director, Dr. Mike Naldrett to meet ever increasing needs for a range of technical services using mass spectrometry, including identification and relative quantification of proteins and several advanced methods for profiling and quantitation of small molecules.
The primary aim of the PMF is to provide high quality service and collaborative work in the fields of Proteomics and Metabolomics to support scientists and students within our University community and around the world. Through this we strive to advance world-class research, support grant proposals, assist in the education of future scientists and support economic and scientific advancements. We offer both Full-Service and Self-Service options to meet the unique needs, time requirements and budgets of our users. Our outreach and education program includes workshops, seminars and courses.
Proteomics services include:
Bottom-up Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels.
Quantification by label-free or multiplexed labeling techniques (eg TMT-10/16plex) with microscale subfractionation to increase coverage.
Post Translational Modification Characterization especially phosphopeptide enrichment from whole organism digests, but also ubiquitylation, acetylation, etc.
Metabolomics services include:
Untargeted Quantification of a wide range of small molecules
Primary metabolism using GC-MS and NIST/Fiehn Libraries
Secondary metabolism using LC-MS and Progenesis QI, NIST Library, mzCloud
Targeted Quantification using HPLC or LCMS.
Follow us on TWITTER: https://twitter.com/PMF_UNLBiotech
Services are offerred outside of University of Nebraska–Lincoln
Consulting is offerred outside of University of Nebraska–Lincoln