The CoreMarketplace: Proteomics Resource Center

Flow Cytometry Resource Center (FCRC) (Flow Cytometry) RRID:SCR_017694	Rockefeller University New York, NY 10065	Cell Sorting, Flow Cytometric Analysis, Spectral Flow Cytometry, Data Analysis, Data Analysis And Interpretation, Flow Cytometry Data Analysis	Last Updated: 09/11/2023
Fisher Drug Discovery Resource Center (High Throughput Screening) RRID:SCR_020985	Rockefeller University New York, NY 10065	High-throughput Screening, Circular Dichroism (CD), Fragment-based Screening, Protocol Development, Surface Plasmon Resonance, Isothermal Titration Calorimetry, Biochemical Analysis, Automated Liquid Handling, Drug Discovery	Last Updated: 12/23/2022
RU Bio-Imaging Resource Center (Microscopy (Electron, Fluorescence, Optical)) RRID:SCR_017791	Rockefeller University New York, NY 10065	Cell Imaging, Confocal Microscopy, Laser Capture Microdissection, Multiphoton Microscopy	Last Updated: 12/22/2022
The Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center (Microscopy (Electron, Fluorescence, Optical)) RRID:SCR_021146	Rockefeller University New York, NY 10065	Cryo-Electron Microscopy	Last Updated: 12/22/2022
Proteomics Resource Center (Proteomics) RRID:SCR_017797	Rockefeller University New York, NY 10065	Mass Spectrometry, Metabolomics Platform, Peptide Synthesis, Proteomics, Metabolomics	Last Updated: 12/22/2022
Precision Instrumentation Technologies (Machine Shop) RRID:SCR_020989	Rockefeller University New York, NY 10065	Shared Instrumentation Oversight & Maintenance	Last Updated: 12/22/2022
Genomics Resource Center (Genomics / Genome Analysis and Technologies) RRID:SCR_020986	Rockefeller University New York, NY 10065	Data Analysis, DNA Analysis, Genomics, Microarray, RNA analysis	Last Updated: 12/22/2022
Electron Microscopy Resource Center (Microscopy (Electron, Fluorescence, Optical)) RRID:SCR_017792	Rockefeller University New York, NY 10065	Cryo-Electron Microscopy, Electron Microscopy	Last Updated: 12/22/2022
CRISPR and Genome Editing Resource Center (Transgenics) RRID:SCR_023097	Rockefeller University New York, NY 10065	CRISPR/Cas Project Design, Genotyping	Last Updated: 12/22/2022
Antibody and Bioresource Core Facility (Antibody Development) RRID:SCR_017847	Rockefeller University New York City, NY 10065	BioBanking, Cell Culture, Monoclonal Antibody	Last Updated: 12/20/2022
Transgenic and Reproductive Technology Center (Transgenics) RRID:SCR_023098	Rockefeller University New York, NY 10065	Transgenics	Last Updated: 12/20/2022
Laboratory of Comparative Pathology (Pathology)	Rockefeller University New York, NY 10065	Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI), Histology, Necropsy	Last Updated: 12/20/2022

Rockefeller University

1230 York Avenue

Box 105

New York, NY 10065

United States of America

https://www.rockefeller.edu/proteomics/

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Primary Contact:

Henrik Molina

Last Updated: 12/22/2022

Facility RRID

RRID:SCR_017797

Facility Details

The Proteomics Resource Center (PRC) at The Rockefeller University offers analysis of biomolecules and peptide synthesis for The Rockefeller University community on a subsidized, cost-recovery basis. The Center is also available to other not-for-profit institutions. The Center is located in the south eastern part of The Rockefeller University campus in The Rockefeller Research Building: RRB157. The Center is guided by and reports to a Scientific Advisory Committee (SAC) on a regularly basis.

The PRC team members are analytical chemists with expertise in proteomics, metabolomics and peptide chemistry. The corner stone of the Center is liquid chromatography (LC) and mass spectrometry (MS) which we apply to proteomics, metabolomics, lipid and small molecule analysis – see examples of projects analyzed by the Center.

The Center is equipped with nano-flow as well as higher flow LC. The majority of our instruments are of the Orbitrap type which offer high mass accuracy and high resolution. A triple quadrupole mass spectrometer is also available. The Center is equipped with, and has expertise in using, a wide array of software for data analysis. A full list of hardware and software are available here. Selected instruments housed in the Center are available for walk-up use.

The goal of the Proteomics Resource Center is to extend the limits of sensitivity and broaden the range of tools provided to investigators by implementing and developing novel and useful analytical techniques. To achieve the above we operate in a boutique style, encouraging the PRC scientists and users to collaborate to create a tailored approach to fit the many unique questions that can be answered using our tools and expertise. On a yearly basis, the Center is working with approximately 200 individual users and handles 500+ sample requests making the Center the to-go-to place to discuss proteomic concepts, experiments and data analysis

Facility Policies

Services are offerred outside of Rockefeller University

Consulting is offerred outside of Rockefeller University

Services offered:

Mass Spectrometry

Metabolomics

Metabolomics Platform

Peptide Synthesis

Proteomics

Facility Equipment

Agilent 5977B GC/MSD

GC/MSD is used for environmental, chemical, petrochemical, food, forensic, pharmaceutical, and material testing. [Product Link]

RRID:SCR_019420

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Thermo LTQ Orbitrap XL ETD Hybrid Ion Trap-Orbitrap Mass Spectrometer

Thermo Scientific LTQ Orbitrap XL ETD Hybrid Ion Trap-Orbitrap Mass Spectrometer can be used to identify, characterize and quantify proteins and peptides using. This instrument is a comprehensive solution for a range of proteomics applications including complex PTM analysis; intelligent sequencing of peptides; top-down and middle-down analysis; label-free quantitation; or quantitation using stable isotope labeling methods such as TMT and iTRAQ. [Product Link]

RRID:SCR_020549

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Thermo LTQ XL Linear Ion Trap Mass Spectrometer

Ion trap mass spectrometer with Agilent 1100 HPLC system is used for a variety of studies including metabolite identification, stability testing, degradation products, and high-abundance quantitation. [Product Link]

RRID:SCR_020554

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Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the Thermo Scientific Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer. The Q Exactive HF system combines a state-of-the-art segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Identify, quantify and confirm in a single analysis with a single instrument with the Q Exactive HF mass spectrometer. [Product Link]

RRID:SCR_020558

Citation IDs: S10OD025271

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Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer has increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies. [Product Link]

RRID:SCR_020556

Citation IDs: S10OD021486

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Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Spectrometer performs analysis including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, and MSn analysis of small molecules. System incorporates the brightest ion source, segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology, and ETD HD. [Product Link]

RRID:SCR_020562

Citation IDs: S10OD025242

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Publications

Publications associated with this facility (Click To View):

1.) Conti BA, Ruiz PD, Broton C, Blobel NJ, Kottemann MC, Sridhar S, Lach FP, Wiley TF, Sasi NK, Carroll T, Smogorzewska A (2024 Mar 2). RTF2 controls replication repriming and ribonucleotide excision at the replisome Nature Communications, 15(), 1943. . PMCID: 10908796.

2.) Clark JF, Soriano P (2024 Feb 16). Diverse Fgfr1 signaling pathways and endocytic trafficking regulate early mesoderm development bioRxiv, (), 2024.02.16.580629. . PMCID: 10888970.

3.) Sanghai ZA, Piwowarczyk R, Broeck AV, Klinge S (2023 May). A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation. Nature structural & molecular biology, 30(5), 594-599. . PMID: 37037974.

4.) Sanghai ZA, Piwowarczyk R, Broeck AV, Klinge S (2023 Apr 10). A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation Nature Structural & Molecular Biology, 30(5), 594-599. . PMCID: 10191850.

5.) Berman AY, Wieczorek M, Aher A, Olinares PDB, Chait BT, Kapoor TM (2023 Mar 6). A nucleotide binding-independent role for γ-tubulin in microtubule capping and cell division. The Journal of cell biology, 222(3), . . PMID: 36695784.

6.) Harper NJ, Burnside C, Klinge S (2023 Feb). Principles of mitoribosomal small subunit assembly in eukaryotes. Nature, 614(7946), 175-181. . PMID: 36482135.

7.) Berman AY, Wieczorek M, Aher A, Olinares PD, Chait BT, Kapoor TM (2023 Jan 25). A nucleotide binding–independent role for γ-tubulin in microtubule capping and cell division The Journal of Cell Biology, 222(3), e202204102. . PMCID: 9930161.

8.) Hsu DJ, Gao J, Yamaguchi N, Pinzaru A, Wu Q, Mandayam N, Liberti M, Heissel S, Alwaseem H, Tavazoie S, Tavazoie SF (2023 Jan 6). Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells Science Advances, 9(1), eade9120. . PMCID: 9821863.

9.) Hsu DJ, Gao J, Yamaguchi N, Pinzaru A, Wu Q, Mandayam N, Liberti M, Heissel S, Alwaseem H, Tavazoie S, Tavazoie SF (2023 Jan 6). Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells. Science advances, 9(1), eade9120. . PMID: 36608131.

10.) Choi CH, Barr W, Zaman S, Model C, Park A, Koenen M, Lin Z, Szwed SK, Marchildon F, Crane A, Carroll TS, Molina H, Cohen P (2022 Nov 8). LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation eLife, 11(), e81559. . PMCID: 9674348.

11.) Choi CHJ, Barr W, Zaman S, Model C, Park A, Koenen M, Lin Z, Szwed SK, Marchildon F, Crane A, Carroll TS, Molina H, Cohen P (2022 Nov 8). LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation. eLife, 11(), . . PMID: 36346018.

Associations

Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=443
https://coremarketplace.org/RRID:SCR_017797

Institution

Institution ROR ID: https://ror.org/0420db125

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

Analysis, synthesis, biomolecule, protein, peptide, mass, spectrometry, protomic, multiple, reaction, monitoring, selected, ion, SIM, MRM, stable, isotope, labeled, residue, service, core

Resource Type:

access service resource, service resource, core facility

Citation:

Rockefeller University Proteomics Resource Center Core Facility (RRID:SCR_017797)

Proteomics Resource Center (Proteomics)

Primary Contact:

Facility RRID

Facility Details

Facility Policies

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Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

Contact Henrik Molina

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Proteomics Resource Center

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