10X Genomics Chromium Plus Genetic Analyzer

Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]

RRID:SCR_019326

Citation IDs: P20GM130454,S10OD025235

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2100 Bioanalyzer Instrument

The 2100 Bioanalyzer system is an automated electrophoresis tool that provides precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use. [Product Link]

RRID:SCR_018043

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ABI 7300 Real-Time Instrument

Fast Real-Time PCR System uses fluorescent based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and dissociation-curve analysis. [Product Link]

RRID:SCR_019335

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Applied Biosystems 3730 Genetic Analyzer

The 48-capillary 3730 DNA Analyzer is a medium-to-high throughput genetic analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. This is upgradeable to 96 capillaries. [Product Link]

RRID:SCR_018052

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Applied Biosystems 3730xl Capillary Genetic Sequencer

The Applied Biosystems 3730xl is a 96-capillary DNA Analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less. Multiple automation features decrease costly human errors. Optimized polymers increase your productivity without compromising your results. Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation. [Product Link]

RRID:SCR_018059

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Applied Biosystems 7900HT Fast Real-Time PCR System

The Applied Biosystems 7900HT is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. The Applied Biosystems 7900HT Fast Real-Time PCR System (7900HT Fast System) uses fluorescent-based PCR chemistries to provide: Quantitative detection of nucleic acid sequences using real-time analysis and Qualitative detection of nucleic acid sequences using end-point and dissociation-curve analysis. [Product Link]

RRID:SCR_018060

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Beckman Coulter Biomek FX Liquid Handler

Beckman Coulter Biomek NXp Robotic Liquid Handler is and uatomated liquid handler designed to handle every aspect of liquid handling, pipetting, dilution, dispensing and integration in to one compact unit that will let you add laboratory automation where you need it. This efficient robotic liquid handler is set with a Span-8 pipette model, 4 fixed tip heads and 4 disposables, with rotating gripper and 8 channel liquid level sensing for an efficient and hands-free workflow. This automated workstation has up to 12 deck positions, automated labware positioners, and labware stacking for on deck storage. The open-ended flexibility of this robotic handler helps keep your lab agile and adaptable to your needs. The Beckman Coulter Biomek NXp Automated Workstation has a dispensing volume of between 0.5 ?L - 5,000 ?L and supports labware from tubes to 1,536-well microplates with accurate intra-well pipetting. The Biomek NXP can integrate with third party devices, and is configurable to meet specific applications needs. This automated workstation is computer controlled through the Biomek software, a robust suite that compliments this efficient instrument with methods and capabilities for genomic, drug discovery & basic research workflows. The Biomek software guides users through set up and protocols, quickly and easily through an icon based interface, with data tracking and LIMS connectivity. [Product Link]

RRID:SCR_019625

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Biotek FLX800 Multi-Detection Microplate Reader

Microplate reader that includes several models with options that meet specific needs of research and OEM users. Top and bottom reading are available to read anything from 6 well to 384 well microplates, and PCR plates. Optional external dispenser module allows running applications such as ion channel assays and flash luminescence assays. [Product Link]

RRID:SCR_019743

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BioTek MultiFlo FX Multi-Mode Dispenser

Automated multi-mode reagent dispenser for 6- to 1536-well microplates. [Product Link]

RRID:SCR_019746

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BioTek Synergy HT Plate Reader

Luminescent assays are sensitive and quantitative tools commonly used for a variety of purposes in biomedical and pharmaceutical research. Quantitation of ATP can be used to detect and measure cellular growth, as well as bacterial contamination. Luminescent genetic reporting assays are widely used to study gene expression and cellular responses to external stimuli in Prokaryotic and Eukaryotic organisms. Dual-reporter assays use two independent reporter systems simultaneously to improve experimental accuracy. One reporter is used to measure the response resulting from the experimental conditions and is often referred to as the “experimental� reporter. The second reporter is designed not to respond to the experimental conditions, acting as an internal control from which data generated by the experimental reporter can be normalized. Normalization of the data serves to compensate for variability caused by differences in transfection efficiency, cell viability, cell lysis, and pipetting. Promega's Dual-Luciferase System uses the activities of luminescent proteins (luciferases) from the firefly (Photinus pyralis) beetle and the sea pansy (Renilla renformis) to serve as an experimental and a control reporter respectively (See Figure 1). The Synergy HT Multi-Detection Reader (BioTek Instruments, Winooski, VT) is a robotic compatible microplate reader that can measure absorbance, fluorescence, and luminescence in all plate formats up to 384-well plates with performance in all three detection modes, that has recently been optimized for luminescence measurements (Figure 2). The Synergy HT utilizes a dual optics design that has both a monochromator/xenon flash system with a silicone diode detector for absorbance and a tungsten halogen lamp with blocking interference filters and a photomultiplier tube (PMT) detector for fluorescence. Reagent addition is accomplished by an optional external injector module, which controls two independent injector syringes. Each syringe is connected to a separate injector tip. Emitted luminescence is captured using either the top or bottom probes and measured using the Synergy HT's low noise PMT operated in photon integration mode. Here we describe the use of a Synergy HT Multi-Detection Microplate Reader (BioTek Instruments) to perform dual-luciferase measurements with purified recombinant enzymes. [Product Link]

RRID:SCR_020536

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Illumina MiSeq System

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]

RRID:SCR_020134

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Illumina NextSeq 550 System

With tunable output and high data quality, it provides flexible power you need for whole genome, transcriptome, and targeted resequencing plus ability to scan microarrays including Infinium MethylationEPIC BeadChip and other select BeadChips. [Product Link]

RRID:SCR_020141

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Roche Light Cycler 480 Instrument

LightCycler 480 Instrument is a real-time PCR device, allowing the use of additional thermal block cycler units (96-well and 384-well format) with the system. The LightCycler 480 Block Kit 384 Silver comprises a block cycler unit for 384-well PCR plates, a corresponding block lid for the 384-well PCR plates, and a storage box for both accessories. [Product Link]

RRID:SCR_020502

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Thermo Scientific Nanodrop 1000 Spectrophotometer

The Thermo Scientific NanoDrop™ 1000 is a spectrophotometer that performs measuring of 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer). [Product Link]

RRID:SCR_018035

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