The UC Proteomics Laboratory (UCPL) is fully equipped with the protein separation and mass spectrometry equipment as well as the trained personnel to perform comparative analyses of complex protein mixtures and to identify the differentially-expressed proteins. Proteomics laboratory personnel also offer guidance to the research community regarding the feasibility of a given approach and/or advice on proteomics experimental design to enhance the probability of success. Please contact the lab director to set up a consultation.

The research expertise in the UC Proteomics Laboratory provides the identification of differentially expressed proteins and the characterization of protein modifications (e.g. phospho, ubiquitin, glyco). Furthermore, various heavy isotope tagging (e.g. TMT) and label-free methods are used to both identify the proteins and quantify the changes in abundance. Additional research-oriented projects include methods to quantify global changes in modifications from cell and tissues, proximity labeling (e.g., APEX/bioID) profiling, and mass spectrometry-based methods quantitation for proteins via surrogate peptides using SRM/MRM methodology.

The Genomics, Epigenomics, and Sequencing Core (GES Core), originally established as the Genomics and Microarray Laboratory in 1999, was restructured in 2012 as a facility focused on next-generation sequencing. The core provides comprehensive services to investigators at the University of Cincinnati, Cincinnati Children’s Hospital Medical Center, as well as external institutions nationwide.

Since its transition, the core has remained fully self-sustaining and has consistently maintained strong financial performance. It is currently among the best-equipped laboratories within the College of Medicine, offering advanced capabilities in next-generation sequencing, single-cell sequencing, and a broad range of genomics and epigenomics services.

The Department of Cancer Biology houses the Advanced Cell Analysis Service Center (ACASC) which offers access to a variety of microscopes and flow cytometry equipment for researchers.

Services offered:
Upright and inverted widefield microscopy
Confocal Microscopy (Zeiss LSM 710)
Super-Resolution Microscopy (Nikon SIMe)
Live Cell Microscopy (Incucylte)
Flow Cytometry (BD LSR Fortessa)
Cell Sorting (BD Aria III)

Established in July 2022, the UC Center for Advanced Structural Biology (CASB) is integrating the latest technology in single-particle cryogenic electron microscopy (cryo-EM). A Thermo Scientific Talos L120C Transmission Electron Microscope (TEM) is available for visualizing nanoparticles, negatively stained proteins or conventional biological samples. It can be operated at 80kV or 120kV.
A Thermo Scientific Glacios 200 kV cryogenic TEM is equipped with both a Ceta-16M camera and a TFS Falcon 4 direct electron detector, in addition to a Selectris Energy Filter. This system is able to generate images for atomic resolution structures for single particle analysis projects. The Glacios also includes software for micro-Electron Diffraction data set collection.

Services Available:
Training and access to the Mark IV Vitrobot plunge freezer
Training and access to the Talos L120C TEM system
Training and access to the Glacios 200 kV cryogenic TEM system
Assisted TEM analysis by CASB personnel (inquiry required)

We provide expertise and support for sample collection, processing, storage and distribution.